Understanding the Influence of Applying Two Culture Filtrates to Control Gray Mold Disease (Botrytis cinerea) in Tomato

Botrytis cinerea, a causal agent of gray mold disease, is one the most destructive fungal pathogens that leads to substantial global economic crop losses, especially for tomato plants. The present study aims investigate inhibitory effect two microbial culture filtrates (BCA filtrate alone and combined with salicylic acid) Trichoderma longibrachiatum Pseudomonas sp. against phytopathogenic fungus B. cinerea on biochemical modifications, disease incidence, fruit quality parameters tomatoes were determined according tested treatments. results showed both fungi bacteria able solubilize phosphate produce IAA HCN. T. could hydrolytic enzymes (chitinase, protease, glucanase). Otherwise, capacity catalase amylase enzymes. Both inhibited hyphae growth cinerea. biocontrol efficacy, in vitro, was significant: up 50% terms inhibition rate at concentration 40%. seedlings’ promoted by separate preventive treatments each micro-organism filtrate. In addition, severity seedlings significantly reduced. Furthermore, treatment acid induced significant physiological changes firmness, juice yield, total protein, ROS enzyme activities. can be recommended as an effective biofungicide control under storage conditions.